Enhancing epi ‐cedrol production in Escherichia coli by fusion expression of farnesyl pyrophosphate synthase and epi ‐cedrol synthase
نویسندگان
چکیده
منابع مشابه
Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum.
A clone encoding farnesyl diphosphate synthase (FPPS) was obtained by PCR from a cDNA library made from young leaves of Artemisia annua. A cDNA clone encoding the tobacco epi-aristolochene synthase (eAS) was kindly supplied by J. Chappell (University of Kentucky, Lexington, KY, USA). Two fusions were constructed, i.e. FPPS/eAS and eAS/FPPS. The stop codon of the N-terminal enzyme was removed an...
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We report the cloning and sequencing of a gene encoding the farnesyl pyrophosphate synthase of Trypanosoma cruzi. The protein (T. cruzi farnesyl pyrophosphate synthase, TcFPPS) is an attractive target for drug development, since the growth of T. cruzi is inhibited by carbocation transition state/reactive intermediate analogs of its substrates, the nitrogen-containing bisphosphonates currently i...
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Bisphosphonates (BPs) are the most widely used and effective treatment for osteoporosis and Paget's disease. Non-nitrogen containing BPs (non-N-BPs), namely etidronate, clodronate, tiludronate, as well as nitrogen containing BPs (N-BPs), namely pamidronate, alendronate, ibandronate, risedronate, zoledronate and minodronate have been launched on the market to date. N-BPs act by inhibiting the en...
متن کاملcDNA cloning and expression analysis of farnesyl pyrophosphate synthase from Ornithogalum saundersiae.
Farnesyl pyrophosphate synthase (FPPS, EC 2.5.1.10) catalyzes the consecutive head-to-tail condensations of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP) to form farnesyl pyrophosphate (FPP), a key precursor of sesquiterpenoids, triterpenoids, sterols, and farnesylated proteins. Here we report the molecular cloning and functional identification of a new full-length cDNA e...
متن کاملApplications of Epi-Retractable and Co-Epi-Retractable Modules
A module M is called epi-retractable if every submodule of M is a homomorphic image of M. Dually, a module M is called co-epi-retractable if it contains a copy of each of its factor modules. In special case, a ring R is called co-pli (resp. co-pri) if RR (resp. RR) is co-epi-retractable. It is proved that if R is a left principal right duo ring, then every left ideal of R is an epi-retractable ...
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ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2019
ISSN: 1618-0240,1618-2863
DOI: 10.1002/elsc.201900103